Generation of CNPY3 knock out cell line in the H1 (WA01) hESC background
Summary
The CNPY3 gene encodes a protein that interacts with members of the toll-like receptor (TLR) protein family and functions as a chaperone, aiding in the proper folding and export of these proteins. We generated a homozygous CNPY3 knockout human embryonic stem cell (hESC) line WAe001-A-2T (H1-CNPY3-/-), using CRISPR/Cas9 genome editing technology. The WAe001-A-2T cell line exhibited a normal karyotype and maintained the typical characteristics of undifferentiated hESCs, including pluripotent gene expression and differentiation potential in vivo. The CNPY3 knockout cell line serves as a valuable resource for investigating the role of the CNPY3 gene in embryonic development and lineage differentiation in vitro. Copyright © 2025. Published by Elsevier B.V.
| Authors | Qi X, Yao F, Yongli S, Xiaofen Z |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2025 Feb 11;84:103676 |
| PubMed | 39961165 |
| DOI | 10.1016/j.scr.2025.103676 |