Applications of piggyBac Transposons for Genome Manipulation in Stem Cells


Transposons are mobile genetic elements in the genome. The piggyBac (PB) transposon system is increasingly being used for stem cell research due to its high transposition efficiency and seamless excision capacity. Over the past few decades, forward genetic screens based on PB transposons have been successfully established to identify genes associated with drug resistance and stem cell-related characteristics. Moreover, PB transposon is regarded as a promising gene therapy vector and has been used in some clinically relevant stem cells. Here, we review the recent progress on the basic biology of PB, highlight its applications in current stem cell research, and discuss its advantages and challenges. Copyright © 2021 Yi Sun et al.

Authors Sun Y, Liu G, Huang Y
Journal Stem cells international
Publication Date 2021;2021:3829286
PubMed 34567130
PubMed Central PMC8460389
DOI 10.1155/2021/3829286

Research Projects

Cell Lines