WAe001-A-96

H1X-10/H1.0-31, H1X10/H1.031

General

Cell Line

hPSCreg Name
WAe001-A-96
Alternative name(s)
H1X-10/H1.0-31, H1X10/H1.031
Cell line type Human embryonic stem cell (hESC)
Last update 11th October 2022
Notes Linker histones H1X/H1.0 double knockout. The H1.0 linker histone was knocked out from WAe001-A-78 (H1X single knockout) subclone.
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Provider

Generator King Abdullah University of Science and Technology (KAUST)
Owner King Abdullah University of Science and Technology (KAUST)
Distributors
Derivation country Saudi Arabia

External Databases

BioSamples SAMEA111453859
CLO CLO_0104105

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA7767418

Ethics

Also have a look at the ethics information for the parental line WAe001-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe001-A.

Culture Conditions

Surface coating Laminin
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
OCT4
Yes
OCT3
Yes
Morphology pictures
morphology H1X-10-H1.0-31.pdf
pluripotent morphology
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Morphology
Protocol or reference
differentiation protocol.pdf
direct differentiation protocol
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Brachyury
Yes
NCAM
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Nestin
Yes
SOX1
Yes
CD44
Yes
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: +12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
H1.0 (target)
Gene knock-out
Chromosome 22: 37,805,229-37,807,432
The knockout was genotyped by Sanger sequencing, RT-qPCR, and Western blot to confirm the absence of the sequence/protein. This knockout was derived from the already knocked out H1X-10 cell line (WAe001-A-78)
CRISPR-associated (CRISPR/Cas) System
H1.X-10 genotyping.pdf
First knockout confirmation - H1X
H1X-10H1.0-31 genotyping (1).pdf
Second knockout confirmation - H1.0