Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing
Summary
The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wu F, Gao G, Pan T, Yang Z, Xu G, Abbas N, Liu Y, Chen Y, Tan S, You K, Ke X, Zhuang Y, Lin X, Yang F, Li YX |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 Mar;27:5-9 |
| PubMed | 29278761 |
| DOI | 10.1016/j.scr.2017.12.002 |