Aspects of zone-like identity and holotomographic tracking of human stem cell-derived liver sinusoidal endothelial cells
Summary
Holotomography and developed machine learning-based algorithm for image processing allowed us to describe and monitor changes in intracellular pore-like structures over time. Finally, we tested the possibility of inducing aspects of zone-specific LSEC phenotype and metabolism using culture-based treatments, which resulted in modest shifts in marker expression and metabolic activity. The presented strategy provides an advanced tool kit for investigating liver endothelial cells. Copyright © 2025 Amirola-Martinez, Combriat, Ferencevic, Wilhelmsen, Dalmao-Fernandez, Olsen, Stokowiec, Aizenshtadt and Krauss.
| Authors | Amirola-Martinez M, Combriat T, Ferencevic K, Wilhelmsen I, Dalmao-Fernandez A, Olsen PA, Stokowiec J, Aizenshtadt A, Krauss S |
|---|---|
| Journal | Frontiers in cell and developmental biology |
| Publication Date | 2025;13:1528991 |
| PubMed | 41181706 |
| PubMed Central | PMC12575375 |
| DOI | 10.3389/fcell.2025.1528991 |