Generation of a SV2A knockout human embryonic stem cell line by CRISPR/Cas9 system
Summary
Synaptic Vesicle Glycoprotein 2A (SV2A) is a ubiquitously expressed brain glycoprotein, localized to synaptic terminals. It regulates vesicle exocytosis, maintains neurotransmitter release, and serves as a receptor for both botulinum neurotoxins (e.g., BoNT/A) and tetanus neurotoxin (TeNT). It is a target for antiseizure drugs and implicated in epilepsy, Alzheimer's, and Parkinson's diseases. We generated a homozygous SV2A-knockout human embryonic stem cell (hESC) line WAe001-A-3F (H1-SV2A-/-), using CRISPR/Cas9 genome editing technology. The SV2A-knockout embryonic stem cell lines provide a precise in vitro model to dissect its roles in synaptic function and disease mechanisms. Copyright © 2026 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Yao F, Qi X, Yongli S, Xiaofen Z |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2026 Feb 7;91:103924 |
| PubMed | 41679028 |
| DOI | 10.1016/j.scr.2026.103924 |