A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing
Summary
Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and ß-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and ß-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Alexeeva V, Aydin IT, Schaniel C, Stranahan AW, D'Souza SL, Bieker JJ |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 May;45:101837 |
| PubMed | 32413789 |
| PubMed Central | PMC7297435 |
| DOI | 10.1016/j.scr.2020.101837 |