A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing


Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and ß-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and ß-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Alexeeva V, Aydin IT, Schaniel C, Stranahan AW, D'Souza SL, Bieker JJ
Journal Stem cell research
Publication Date 2020 May;45:101837
PubMed 32413789
PubMed Central PMC7297435
DOI 10.1016/j.scr.2020.101837

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