Generation of WAe001-A-15, a human embryonic stem cell line with miR-122 doxycycline-inducible expression
Summary
MiR-122 is the most abundant miRNA in the human liver accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 is a valuable therapeutic target for liver diseases, including viral hepatitis, fibrosis, steatosis, and hepatocarcinoma. Here, we constructed a miR-122 doxycycline-inducible expression human embryonic stem cell line WAe001-A-15 using the piggyBac transposon system. The cell line retained its pluripotency, in vitro differentiation potential, normal morphology, and karyotype. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Liu Y, Wu F, Liu D, Wei R, Abbas N, Xia J, Li D, Wang H, Zhuang Y, Guo D, Chen Y, Wu Y, Ke X, Tao J, Yang F, Lai K, Shu X, Li YX |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 May;29:84-87 |
| PubMed | 29627726 |
| DOI | 10.1016/j.scr.2018.03.007 |