Generation of two H1 hESC sublines carrying a heterozygous and homozygous knock-out of RB1


Retinoblastoma is a childhood cancer of the retina caused by biallelic inactivation of the tumor suppressor gene RB1. In heritable retinoblastoma, one allele is inherited in mutant form via one of the parental germ cells. To study molecular mechanisms in retinoblastoma, two sublines of H1 hESCs were generated, carrying a knock-out allele of RB1 in the heterozygous or homozygous state. Exon 3 of RB1 was targeted and modified by nucleotide deletions using the CRISPR/Cas9 nuclease system. Based on a nearby single nucleotide polymorphism, the modification could be assigned to one allele. Copyright © 2017 The Author. Published by Elsevier B.V. All rights reserved.

Authors Steenpass L
Journal Stem cell research
Publication Date 2017 Dec;25:270-273
PubMed 29246572
DOI 10.1016/j.scr.2017.07.005

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