Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system

Summary

Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) line using the CRISPR/Cas9 genome-editing technology. We believe that this cell line will be a good resource for exploring the function of the MEIS1 gene in embryonic development in vitro. Furthermore, the gene-knockout method reported in this study is efficient and labor-saving, which may provide an effective strategy for hESC gene deletion. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang C, Yu Y, Li F, Lan X, Wang L
Journal Stem cell research
Publication Date 2020 Oct 29;49:102069
PubMed 33157390
DOI 10.1016/j.scr.2020.102069

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