Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines


Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Sims CHC, Autio MI, Buskin A, Cheung C, Heer R, Foo RSY, Wang X
Journal Stem cell research
Publication Date 2021 Apr;52:102238
PubMed 33611044
DOI 10.1016/j.scr.2021.102238

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