Generation of FOXJ1-EGFP knock-in reporter human embryonic stem cell line, WAe001-A-2D, using CRISPR/Cas9-based gene targeting
Summary
Forkhead box protein J1 (FOXJ1), a member of the forkhead family, is an important transcription factor regulating multiciliated cell differentiation and motile ciliogenic program. Here, we established a FOXJ1- EGFP knock-in human embryonic stem cell (hESC) line by inserting a P2A-EGFP gene cassette of FOXJ1 using CRISPR/Cas9 system. The reporter cell line retained a normal karyotype, expressed comparable pluripotent marker genes, and maintained differentiation potential. This reporter cell line enables live identification of multiciliated cells during the general lung differentiation and will be a valuable tool for studying the multiciliated cell differentiation, ciliogenesis and mechanism of related pulmonary diseases. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Chen J, Dai T, Li Q, Xu T, Zhang W, Sun J, Liu H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Aug;78:103445 |
| PubMed | 38820864 |
| DOI | 10.1016/j.scr.2024.103445 |