Establishment of an ectodermal dysplasia related gene EDA Knockout human embryonic stem cell line (WAe001-A-22) by CRISPR-Cas9 technology


EDA is a gene located at Xq13.1. It encodes different isoforms of tumor necrosis factor (TNF) superfamily member ectodysplasin A. Ectodysplasin A is a transmembrane protein which can be cleaved to form a secreted form and interact with EDA receptor to mediate the development of ectoderm. Mutations of the EDA gene are related to ectodermal dysplasia and tooth agenesis. Here, we report the establishment of the EDA gene knockout human embryonic stem (hES) cell line by CRISPR-Cas9 technology. This cell line provides good materials for further studies of the roles ectodysplasin A plays in ectoderm differentiation and tooth development. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Xue Y, Liao B, Xie Y, Li S, Ma X, Sun X
Journal Stem cell research
Publication Date 2019 Jan;34:101379
PubMed 30605838
DOI 10.1016/j.scr.2018.101379

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