hPSCreg®
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WAe001-A-3T

Registration Summary:
The cell line is not validated yet.
WAe001-A-3T

General

Cell Line
hPSCreg name WAe001-A-3T
Cite as:
WAe001-A-3T
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 20th January 2026
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Provider
Generator Institute for Cardiovascular Science of Soochow University (ICSSU)

External Databases
BioSamples SAMEA120930534

General Information
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex male
Ethnicity N/A

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)
BioSamples SAMEA7767418

Ethics

Also have a look at the ethics information for the parental line WAe001-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe001-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
REPS2 (target)
Gene knock-out
Xp22.2
The REPS2 gene (RalBP1-associated Eps domain-containing protein 2, also known as POB1) interacts with proteins such as RALBP1 and regulates the internalization of growth factor receptors, including EGFR and the insulin receptor, thereby modulating cellular signaling, survival, motility, and proliferation. Alterations in REPS2 expression or function have been associated with multiple disease processes, particularly cancer.
CRISPR-associated (CRISPR/Cas) System