Generation of SFTPC-mCherry knock-in reporter human embryonic stem cell line, WAe001-A-2H, using CRISPR/Cas9-based gene targeting
Summary
The SFTPC gene is responsible for the production of the pulmonary surfactant protein C (SPC), a highly hydrophobic molecule that plays a crucial role in maintaining lung integrity through its influence on the synthesis of alveolar surfactant proteins. In this study, we harnessed the CRISPR/Cas9 system for precise genome editing to create a modified H1 human embryonic stem cell (hESC) line, incorporating the SFTPC-mCherry reporter construct. Therefore, the engineered SFTPC-mCherry knock-in (KI) hESC line can serve as an effective tool for tracking the expression patterns of the SFTPC gene as alveolar type 2 cells differentiate from hESCs. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Sun H, Li Q, Xu T, Zhang W, Sun J, Liu H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Dec;81:103597 |
| PubMed | 39476617 |
| DOI | 10.1016/j.scr.2024.103597 |