WAe001-A-79

H1_CTR1-mEos4b

General

Cell Line

hPSCreg Name
WAe001-A-79
Alternative name(s)
H1_CTR1-mEos4b
Cell line type Human embryonic stem cell (hESC)
Last update 16th June 2022
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Provider

Generator University of Houston - Main Campus (UHMC)

External Databases

BioSamples SAMEA14370583
CLO CLO_0103894
Cellosaurus CVCL_C0C9
Wikidata Q112930550

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA7767418

Ethics

Also have a look at the ethics information for the parental line WAe001-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe001-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Brachyury
Yes
Troponin T
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
β3-Tubulin
Yes
Nestin
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
A total of 22 metaphases were karyotyped by G-banding: 16 showed normal 46, XY. 2 showed 45, XY, t(14q:14q). 1 showed 44,X, -Y, t(14q;14q). 1 showed 45, X, -Y. 1 showed 47, XY, -4, break in centromeric region of chr 4. 1 showed 47, XY, -9, break in centromeric region of chr 9.
Passage number: 11
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
CTR1 (SLC31A1) (target)
Transgene expression
9q32
A photoconvertible fluorescence protein, mEos4b, followed by a flag tag was inserted at the location four amino acids before the HCH domain of CTR1's cytoplasmic tail (Cut locus: chr9:-113260473). The engineered cells express CTR1-mEos4b fusion protein.
CRISPR-associated (CRISPR/Cas) System