Generation of SST-P2A-mCherry reporter human embryonic stem cell line using the CRISPR/Cas9 system (WAe001-A-2C)
Summary
Somatostatin (SST)-producing pancreatic delta-cells play an important role in maintaining the balance of insulin and glucagon secretion within the islets. This study aimed to generate a human embryonic stem cell (hESC) line with a SST-P2A-mCherry reporter using CRISPR/Cas9 system. The SST-P2A-mCherry reporter cell line was shown to maintain typical pluripotent characteristics and able to be induced into SST-producing pancreatic delta-cells. The generation of the cell line would provide useful platform for the characterization of stem cell-derived delta-cells, discovery of delta-cell surface markers and investigation of paracrine mechanisms, which will ultimately promote the drug discovery and cell therapy of diabetes mellitus. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Zhang T, Zhang F, Wang N, Xu T, Zhu L, Chen L, Liu H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Jun;77:103397 |
| PubMed | 38547668 |
| DOI | 10.1016/j.scr.2024.103397 |