Derivation of human embryonic stem cells in defined conditions
Summary
We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
| Authors | Ludwig TE, Levenstein ME, Jones JM, Berggren WT, Mitchen ER, Frane JL, Crandall LJ, Daigh CA, Conard KR, Piekarczyk MS, Llanas RA, Thomson JA |
|---|---|
| Journal | Nature biotechnology |
| Publication Date | 2006 Feb;24(2):185-7 |
| PubMed | 16388305 |
| DOI | 10.1038/nbt1177 |