WA14, H14

General#

Cell Line

hPSCreg Name WAe014-A
Alternative name(s)
WA14, H14
Cell line type Human embryonic stem cell (hESC)
Last update 16th August 2018
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Provider

Generator WiCell Research Institute (WA)
Derivation country United States

External Databases

Cellosaurus CVCL_9775
NIHhESC NIHhESC-10-0064

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

All cell lines of this donor's relatives
Has sister:
Has brother:

Ethics#

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent All original consent documents including consent for IVF, for freeze of materials and for donation has been reviewd and approved by both the US NIH and the UK MRC. US NIH registration number is 0064. UK MRC approval is SCSC11-18 and SCSC06-18
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? IRB approval
Approval number

hESC Derivation#

Date of derivation 1998-01-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Cell isolation Immunosurgery
Cell seeding Isolated ICM
Derivation under GMP?
No

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
FGF 2

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-1
No
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
TDGF1
Yes
Telomerase Activity
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 24

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)