DNAJC19 KO
WAe007-A-3
General
Cell Line |
|
| hPSCreg name | WAe007-A-3 |
| Cite as: | WAe007-A-3 (RRID:CVCL_D0PI) |
| Alternative name(s) |
DNAJC19 KO
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | |
| Last update | 13th September 2023 |
| User feedback | |
Provider |
|
| Generator | National Center for Cardiovascular Diseases & Fuwai Hospital (NCCDFW) |
| Owner | National Center for Cardiovascular Diseases & Fuwai Hospital (NCCDFW) |
| Derivation country | China |
External Databases |
|
| BioSamples | SAMEA114299692 |
| Cellosaurus | CVCL_D0PI |
| Wikidata | Q123033857 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| All cell lines of this donor's relatives |
Has brother:
Has brother:
|
External Databases (Donor) |
|
| BioSamples | SAMEA6407537 |
Ethics
Also have a look at the ethics information for the parental line
WAe007-A
.
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe007-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
|
Differentiation Potency
Neural epithelium
In vivo teratoma
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
|
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