WA13, H13

General#

Cell Line

hPSCreg Name WAe013-A
Alternative name(s)
WA13, H13
Cell line type Human embryonic stem cell (hESC)
Last update 16th August 2018
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Provider

Generator WiCell Research Institute (WA)
Distributors
Derivation country United States

External Databases

Cellosaurus CVCL_9774
NIHhESC NIHhESC-10-0063

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

All cell lines of this donor's relatives
Has sister:
Has brother:

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 1998-01-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Cell isolation Immunosurgery
Cell seeding Isolated ICM
Derivation under GMP?
No

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
FGF 2

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
TDGF1
Yes
Telomerase Activity
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 26

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)