WA13, H13
WAe013-A
General
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Donor Relations |
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| All cell lines of this donor's relatives |
Has sister:
Has brother:
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Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | All original consent documents including consent for IVF, for freeze of materials and for donation has been reviewd and approved by both the US NIH and the UK MRC. US NIH registration number is 0063. UK MRC approval is SCSC06-18 |
| Alternative consent approval number | NIHhESC-10-0063 |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| How may genetic information associated with the cell line be accessed? | |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | University of Wisconsin IRB |
| Approval number | 95-623-239 |
hESC Derivation
| Date of derivation | 1998-01-01 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
| Derivation under GMP? |
No |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Enzymatically and Mechanically | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-1 |
No |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| GABRB3 |
Yes |
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| NANOG |
Yes |
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| TDGF1 |
Yes |
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| Telomerase Activity |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
26
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 75
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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