WA13, H13
WAe013-A
General#
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Donor Relations |
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| All cell lines of this donor's relatives |
Has sister:
Has brother:
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Ethics#
We are currently revising this section.hESC Derivation#
| Date of derivation | 1998-01-01 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
| Derivation under GMP? |
No |
Culture Conditions#
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Enzymatically and Mechanically | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-1 |
No |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| GABRB3 |
Yes |
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| NANOG |
Yes |
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| TDGF1 |
Yes |
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| Telomerase Activity |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
26
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Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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