PHF19-KO
WAe007-A-4
General
iPSC Line |
|
| hPSCreg name | WAe007-A-4 |
| Cite as: | WAe007-A-4 |
| Alternative name(s) |
PHF19-KO
|
| iPSC line type | Human embryonic stem cell (hESC) |
| Similar iPSC lines |
WAe007-A (H7, WA07) WAe007-A-3 (DNAJC19 KO) WAe007-A-5 (H7-AP1-Luciferase-GFP) WAe013-A (WA13, H13) WAe014-A (WA14, H14) |
| Last update | 4th July 2024 |
| User feedback | |
Provider |
|
| Generator |
Wenzhou Medical University (WMU)
Contact:
Wenzhou Medical University (WMU) |
| Owner | Wenzhou Medical University (WMU) |
| Derivation country | China |
External iPSC Databases |
|
| BioSamples | SAMEA115800065 |
General iPSC Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
| Subclone of | |
iPSC Genetic Modification
| Genetic modifications not related to a disease |
|
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| All cell lines of this donor's relatives |
Has brother:
Has brother:
|
External Databases (Donor) |
|
| BioSamples | SAMEA6407537 |
Ethics
Also have a look at the ethics information for the parental line
WAe007-A
.
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe007-A.
|
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
|||||
| POU5F1 (OCT-4) |
Yes |
Differentiation Potency
Neural epithelium
In vivo teratoma
Genotyping
Karyotyping (iPSC Line) |
|
| Has the iPSC line karyotype been analysed? |
Yes
46，XX
Karyotyping method:
G-Banding
|
Other Genotyping (iPSC Line) |
|
Login to share your feedback, experiences or results with the research community.