PHF19-KO

General

Cell Line

hPSCreg name WAe007-A-4
Cite as:
WAe007-A-4
Alternative name(s)
PHF19-KO
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 4th July 2024
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Provider

Generator Wenzhou Medical University (WMU)
Owner Wenzhou Medical University (WMU)
Derivation country China

External Databases

BioSamples SAMEA115800065

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA6407537

Ethics

Also have a look at the ethics information for the parental line WAe007-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe007-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Intestinal Epithelium
Ont Id: NCIT_C49240
In vivo teratoma
Adipose Tissue
Ont Id: NCIT_C12472
In vivo teratoma
Neural epithelium
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
PHF19 (target)
Gene knock-out
CRISPR-associated (CRISPR/Cas) System