General

Cell Line
hPSCreg name	NCCDFWe013-A-3
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	NCCDFWe013-A-3 (RRID:CVCL_D0PI)
Alternative name(s)	DNAJC19 KO
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	13th September 2023
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Provider
Generator	National Center for Cardiovascular Diseases & Fuwai Hospital (NCCDFW) Contact: National Center for Cardiovascular Diseases & Fuwai Hospital (NCCDFW)
Owner	National Center for Cardiovascular Diseases & Fuwai Hospital (NCCDFW)
Derivation country	China
External Databases
BioSamples	SAMEA114299692
Cellosaurus	CVCL_D0PI
Wikidata	Q123033857
General Information
Publications	Chen G et al. Generation of a homozygous DNAJC19 knockout human embryonic stem cell line by CRISPR/Cas9 system. Stem cell research. 2024 Jun;77:103427.
* Is the cell line readily obtainable for third parties?	No
Subclone of	WAe007-A

Donor Information

General Donor Information
Sex	female
Ethnicity	N/A
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Family history	NO
Is the medical history available upon request?	NO
Is clinical information available?	NO
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
Donor Relations
All cell lines of this donor's relatives	Has brother: WAe013-A WAe013-A-1 WISCe014-A-1 WAe013-A-2 WISCe014-A-2 WAe013-A-3 WISCe014-A-3 Has brother: WAe014-A WAe014-A-1 KUCFRe015-A-1 WAe014-A-2 KUCFRe015-A-2
External Databases (Donor)
BioSamples	SAMEA6407537

Ethics

Also have a look at the ethics information for the parental line WAe007-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe007-A.

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
CO2 Concentration	5 %
Medium	mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
SSEA-4	Yes
POU5F1 (OCT-4)	Yes

Differentiation Potency

Endoderm

Intestinal Epithelium
Ont Id: NCIT_C49240

In vivo teratoma

Mesoderm

Adipose Tissue
Ont Id: NCIT_C12472

In vivo teratoma

Ectoderm

Neural epithelium

In vivo teratoma

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46，XX Karyotyping.jpg Karyotyping method: G-Banding
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

Dilated cardiomyopathy with ataxia Synonyms MGA5 3-methylglutaconic aciduria type 5 DCMA syndrome Genetic modifications DNAJC19 (target) Type of modification Gene knock-out Delivery method CRISPR-associated (CRISPR/Cas) System