General

Cell Line
hPSCreg name	ICSSUe008-A-5
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	ICSSUe008-A-5
Alternative name(s)	H7-AP1-Luciferase-GFP
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	23rd December 2024
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Provider
Generator	Institute for Cardiovascular Science of Soochow University (ICSSU) Contact: Institute for Cardiovascular Science of Soochow University (ICSSU)
External Databases
BioSamples	SAMEA115807779
General Information
Publications	Guo X et al. CRISPR/Cas9-mediated generation of AP-1 activity reporter cell line in human embryonic stem cell (WAe007-A-5). Stem cell research. 2024 Dec;81:103557.
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	WAe007-A

Donor Information

General Donor Information
Sex	female
Ethnicity	N/A
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Family history	NO
Is the medical history available upon request?	NO
Is clinical information available?	NO
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
Donor Relations
All cell lines of this donor's relatives	Has brother: WAe013-A WAe013-A-1 WISCe014-A-1 WAe013-A-2 WISCe014-A-2 WAe013-A-3 WISCe014-A-3 Has brother: WAe014-A WAe014-A-1 KUCFRe015-A-1 WAe014-A-2 KUCFRe015-A-2
External Databases (Donor)
BioSamples	SAMEA6407537

Ethics

Also have a look at the ethics information for the parental line WAe007-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe007-A.

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
O2 Concentration	21 %
CO2 Concentration	5 %
Medium	mTeSR™ 1 Supplements Supplement Amount Unit Penicillin-Streptomycin 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe007-A .

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46，XX AP-1-Reporter_Karyotype_analysis-46-XX.TIF Passage number: 10 Karyotyping method: G-Banding
Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease

AAVS1 (target) Type of modification Gene knock-in Chromosome location 19q13 Transgene GFP Chromosome location (transgene) 19q13 Free text A reporter construct was made as follows: [Activator protein 1 (AP-1) binding site fragment] - [TATA-box] - [Luciferase-GFP reporter (LuciGFP)]. This construct was introduced into the AAVS1 locus. Delivery method CRISPR-associated (CRISPR/Cas) System