WA16

General#

Cell Line

hPSCreg Name WISCe002-A
Alternative name(s)
WA16
Cell line type Human embryonic stem cell (hESC)
Last update 20th October 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator University of Wisconsin (WISC)
Derivation country United States

External Databases

Cellosaurus CVCL_9777
NIHhESC NIHhESC-11-0097

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Klinefelter's syndrome
The donor is affected.
Synonyms
  • Klinefelter syndrome
  • XXY syndrome
  • XXY trisomy
  • Hypogonadotropic Hypogonadism
show more synonyms

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Enzymatic
Cell isolation Mechanical

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 10 %
Medium mTeSR™ 1
Supplements
Human Serum Albumin
bFGF
GABA
LiCl
Serum Replacement

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XXY

Other Genotyping (Cell Line)