General

Cell Line
hPSCreg name	WISCe002-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	WISCe002-A (RRID:CVCL_9777)
Alternative name(s)	WA16
Cell line type	Human embryonic stem cell (hESC)
Similar lines	KAUSTi009-A (KS3-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi009-B (KS3-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi010-A (KS5-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi001-B (KS7-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi010-B (KS5-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi006-A (KS6-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi006-B (KS6-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi003-A (KS1-iPSC#D) Donor diseases: Klinefelter's syndrome KAUSTi008-B (KS4-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi008-C (KS4-iPSC#C) Donor diseases: Klinefelter's syndrome KAUSTi008-D (KS4-iPSC#D) Donor diseases: Klinefelter's syndrome KAUSTi008-E (KS4-iPSC#E) Donor diseases: Klinefelter's syndrome KAUSTi007-A (KS2-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi008-F (KS4-iPSC#F) Donor diseases: Klinefelter's syndrome KAUSTi008-G (KS4-iPSC#G) Donor diseases: Klinefelter's syndrome KAUSTi008-A (KS4-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi001-A (KS7-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi007-B (KS2-iPSC#B) Donor diseases: Klinefelter's syndrome KCLe007-A (KCL008_HD2) Donor diseases: Klinefelter syndrome RIe004-A (Royan H4) Donor diseases: Klinefelter syndrome CEBe001-A (FC018) Donor diseases: Klinefelter syndrome
Last update	4th September 2022
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Provider
Generator	University of Wisconsin (WISC) Contact: Madison - Thomson Lab
Derivation country	United States
External Databases
Cellosaurus	CVCL_9777
NIHhESC	NIHhESC-11-0097
Wikidata	Q54993519
General Information
Publications	Ludwig TE et al. Derivation of human embryonic stem cells in defined conditions. Nature biotechnology. 2006 Feb;24(2):185-7.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Klinefelter syndrome The donor is affected. Synonyms Hypogonadotropic Hypogonadism Klinefelter's syndrome XXY syndrome XXY trisomy 47, XXY show more synonyms show less synonyms

Ethics

Alternative consent approval number	NIHhESC-11-0097
How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage	Blastula with ICM and Trophoblast
ZP removal technique	Enzymatic
Cell isolation	Mechanical

Culture Conditions

Surface coating	Matrigel/Geltrex
Passage method	Mechanically
O2 Concentration	5 %
CO2 Concentration	10 %
Medium	mTeSR™ 1 Supplements Supplement Amount Unit Human Serum Albumin bFGF GABA LiCl Serum Replacement

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
NANOG	Yes
POU5F1 (OCT-4)	Yes
ZFP42 (REX-1)	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vivo teratoma

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vivo teratoma

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vivo teratoma

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 47XXY
Other Genotyping (Cell Line)