WA16

General#

Cell Line

hPSCreg Name
WISCe002-A
Alternative name(s)
WA16
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator University of Wisconsin (WISC)
Derivation country United States

External Databases

Cellosaurus CVCL_9777
NIHhESC NIHhESC-11-0097
CLO CLO_0102594
Wikidata Q54993519

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Klinefelter syndrome
The donor is affected.
Synonyms
  • XXY syndrome
  • XXY trisomy
  • Hypogonadotropic Hypogonadism
  • 47, XXY
  • Klinefelter's syndrome
show more synonyms

Ethics#

Alternatives to consent
Alternative consent approval number NIHhESC-11-0097
Name of accrediting authority involved?
Approval number

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Enzymatic
Cell isolation Mechanical

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 10 %
Medium mTeSR™ 1
Supplements
Human Serum Albumin
bFGF
GABA
LiCl
Serum Replacement

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XXY

Other Genotyping (Cell Line)