General

iPSC Line
hPSCreg name	CEBe001-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	CEBe001-A (RRID:CVCL_B349)
Alternative name(s)	FC018
iPSC line type	Human embryonic stem cell (hESC)
Similar iPSC lines	KAUSTi003-A (KS1-iPSC#D) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi008-B (KS4-iPSC#B) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi008-C (KS4-iPSC#C) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi008-D (KS4-iPSC#D) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi008-E (KS4-iPSC#E) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi008-F (KS4-iPSC#F) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi008-G (KS4-iPSC#G) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-A (XXY-A4) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-E (2A6 C11) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-F (2A6 E11) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi001-A (KS7-iPSC#A) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi007-B (KS2-iPSC#B) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi009-A (KS3-iPSC#A) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi009-B (KS3-iPSC#B) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi010-A (KS5-iPSC#A) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi001-B (KS7-iPSC#B) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi010-B (KS5-iPSC#B) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi006-A (KS6-iPSC#A) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-B (XXY-2A1) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-D (2A6 C08) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi006-B (KS6-iPSC#B) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-C (XXY-3A9) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-G (2A6 H06) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-H (3A21) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-I (3A43) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi007-A (KS2-iPSC#A) Donor diseases: obsolete_Klinefelter's syndrome KAUSTi008-A (KS4-iPSC#A) Donor diseases: obsolete_Klinefelter's syndrome KCLe007-A (KCL008_HD2) Donor diseases: Klinefelter syndrome WISCe002-A (WA16) Donor diseases: Klinefelter syndrome CRICKi024-G-1 (2A6 H06_XO) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-H-1 (3A21_XO) Donor diseases: obsolete_Klinefelter's syndrome CRICKi024-I-1 (3A43_XO) Donor diseases: obsolete_Klinefelter's syndrome RIe004-A (Royan H4) Donor diseases: Klinefelter syndrome
Last update	31st August 2022
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Provider
Generator	Takara Bio Europe AB (former Cellartis) (CEB) Contact: Human ES cell line derivation
Derivation country	Sweden
External iPSC Databases
Cellosaurus	CVCL_B349
Wikidata	Q54833302
General iPSC Information
Publications	Heins N et al. Derivation, characterization, and differentiation of human embryonic stem cells. Stem cells (Dayton, Ohio). 2004;22(3):367-76.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	unknown
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Klinefelter syndrome The donor is affected. Synonyms Hypogonadotropic Hypogonadism Klinefelter's syndrome XXY syndrome XXY trisomy 47, XXY show more synonyms show less synonyms
Disease associated phenotypes	Triploid Karyotype

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Date of derivation	2001-09-01
Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	No
ZP removal technique	Enzymatic
Cell isolation	Immunosurgery
Cell seeding	Isolated ICM

iPSC Culture Conditions

Feeder cells	mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040
O2 Concentration	20 %
CO2 Concentration	5 %
Medium	Other medium: Base medium: DMEM/F12 with Glutamax Main protein source: Anti-Human-Serum (Sigma) Serum concentration: % Supplements Supplement Amount Unit Fetal Calf Serum % hrbFGF %

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
TRA 1-60	Yes
TRA 1-81	Yes
POU5F1 (OCT-4)	Yes

PluriTest

Pluripotency Score	Novelty Score	Link to microarray data
17.743	1.158	http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vivo teratoma

In vitro spontaneous differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vivo teratoma

In vitro spontaneous differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vivo teratoma

In vitro spontaneous differentiation

Genotyping

Karyotyping (iPSC Line)
Has the iPSC line karyotype been analysed?	Yes 69XXY Passage number: 58
Other Genotyping (iPSC Line)