KCL008_HD2

General

Cell Line

hPSCreg Name
KCLe007-A
Alternative name(s)
KCL008_HD2
Cell line type Human embryonic stem cell (hESC)
Last update 1st September 2022
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A243
CLO CLO_0102635
Wikidata Q27570645

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Klinefelter syndrome
The donor is affected.
Disease associated phenotypes
  • Triploid Set of Chromosomes

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Date of derivation 2007-04-12
Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
Yes
Expansion status 5
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Mechanical
Cell isolation Laser
Cell seeding Isolated ICM

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
FGF 2 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
68,XXY,-6
Passage number: 24

Other Genotyping (Cell Line)