General

Cell Line
hPSCreg name	KCLe007-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	KCLe007-A (RRID:CVCL_A243)
Alternative name(s)	KCL008_HD2
Cell line type	Human embryonic stem cell (hESC)
Similar lines	KAUSTi009-A (KS3-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi009-B (KS3-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi010-A (KS5-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi001-B (KS7-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi010-B (KS5-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi006-A (KS6-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi006-B (KS6-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi003-A (KS1-iPSC#D) Donor diseases: Klinefelter's syndrome KAUSTi008-B (KS4-iPSC#B) Donor diseases: Klinefelter's syndrome KAUSTi008-C (KS4-iPSC#C) Donor diseases: Klinefelter's syndrome KAUSTi008-D (KS4-iPSC#D) Donor diseases: Klinefelter's syndrome KAUSTi008-E (KS4-iPSC#E) Donor diseases: Klinefelter's syndrome KAUSTi007-A (KS2-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi008-F (KS4-iPSC#F) Donor diseases: Klinefelter's syndrome KAUSTi008-G (KS4-iPSC#G) Donor diseases: Klinefelter's syndrome KAUSTi008-A (KS4-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi001-A (KS7-iPSC#A) Donor diseases: Klinefelter's syndrome KAUSTi007-B (KS2-iPSC#B) Donor diseases: Klinefelter's syndrome WISCe002-A (WA16) Donor diseases: Klinefelter syndrome RIe004-A (Royan H4) Donor diseases: Klinefelter syndrome CEBe001-A (FC018) Donor diseases: Klinefelter syndrome
Last update	1st September 2022
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Provider
Generator	King's College London (KCL) Contact: Department of Women's Health
Derivation country	United Kingdom
External Databases
Cellosaurus	CVCL_A243
Wikidata	Q27570645
General Information
Publications	Stephenson EL et al. Derivation of the King's College London human embryonic stem cell lines. In vitro cellular & developmental biology. Animal. 2010 Apr;46(3-4):178-85.
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Klinefelter syndrome The donor is affected. Synonyms Hypogonadotropic Hypogonadism Klinefelter's syndrome XXY syndrome XXY trisomy 47, XXY show more synonyms show less synonyms
Disease associated phenotypes	Triploid Set of Chromosomes

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Date of derivation	2007-04-12
Embryo stage	Blastula with ICM and Trophoblast
PGD Embryo?	Yes
Expansion status	5 Stephenson 2007
ICM morphology	A Stephenson 2007
Trophectoderm morphology	b Stephenson 2007
ZP removal technique	Mechanical
Cell isolation	Laser
Cell seeding	Isolated ICM

Culture Conditions

Feeder cells	mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040
Passage method	Mechanically
O2 Concentration	5 %
CO2 Concentration	6 %
Medium	Other medium: Base medium: High glucose DMEM Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements Supplement Amount Unit FGF 2 %

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
NANOG	Yes
POU5F1 (OCT-4)	Yes
SSEA-1	No
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
ZFP42 (REX-1)	Yes
SOX2	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 68,XXY,-6 Passage number: 24
Other Genotyping (Cell Line)