KCL008_HD2
KCLe007-A
General
Cell Line |
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hPSCreg name | KCLe007-A |
Cite as: | KCLe007-A (RRID:CVCL_A243) |
Alternative name(s) |
KCL008_HD2
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Cell line type | Human embryonic stem cell (hESC) |
Similar lines |
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Last update | 1st September 2022 |
User feedback | |
Provider |
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Generator |
King's College London (KCL)
Contact:
Department of Women's Health |
Derivation country | United Kingdom |
External Databases |
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Cellosaurus | CVCL_A243 |
Wikidata | Q27570645 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
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Ethics
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Alternative consent approval number | |
How may genetic information associated with the cell line be accessed? |
hESC Derivation
Date of derivation | 2007-04-12 |
Embryo stage | Blastula with ICM and Trophoblast |
PGD Embryo? |
Yes |
Expansion status |
5 |
ICM morphology |
A |
Trophectoderm morphology |
b |
ZP removal technique | Mechanical |
Cell isolation | Laser |
Cell seeding | Isolated ICM |
Culture Conditions
Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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Passage method | Mechanically | ||||||
O2 Concentration | 5 % | ||||||
CO2 Concentration | 6 % | ||||||
Medium |
Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-1 |
No |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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ZFP42 (REX-1) |
Yes |
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SOX2 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
68,XXY,-6
Passage number: 24
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Other Genotyping (Cell Line) |
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