Royan H4

General

Cell Line

hPSCreg Name
RIe004-A
Alternative name(s)
Royan H4
Cell line type Human embryonic stem cell (hESC)
Last update 3rd September 2022
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Provider

Generator Royan Institute (RI)
Derivation country Iran

External Databases

Cellosaurus CVCL_9385
CLO CLO_0102586
Wikidata Q54950987

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Klinefelter syndrome
The donor is affected.
Disease associated phenotypes
  • Triploid Set of Chromosomes

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Surface coating Gelatin
Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal Calf Serum
Serum concentration: 20 %
Supplements
L-Glutamine
LIF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
TRA 1-81
Yes
Connexin43
Yes
SSEA-1
No
Alkaline Phosphatase
Yes
nodal
Yes
ZFP42 (REX-1)
Yes
LEFTY1
Yes
foxd3
Yes
CXCR4
Yes
EPHA2
Yes
NANOG
Yes
PAX6
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TDGF1
Yes
THY1 (CD90)
Yes
PROML1 (AC133)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Mosaic 69XXY

Other Genotyping (Cell Line)