H1.3-64/H1.4-15
WAe001-A-1G
General
Cell Line |
|
| hPSCreg name | WAe001-A-1G |
| Cite as: | WAe001-A-1G (RRID:CVCL_C9L5) |
| Alternative name(s) |
H1.3-64/H1.4-15
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe001-A-E (H1X-10/H1.4-4) WAe001-A-F (H1X-59/H1.4-5) WAe001-A-91 (H1.4-126, H1.4126) WAe001-A-O (H1.0-29/H1.4-14) WAe001-A-92 (H1.4-143, H1.4143) WAe001-A-P (H1.0-102/H1.4-19) WAe001-A-U (H1.1-17/H1.4-18) WAe001-A-V (H1.1-39/H1.4-17) WAe001-A-1A (H1.2-13/H1.4-69) WAe001-A-1B (H1.2-124/H1.4-84) WAe001-A-1H (H1.3-191/H1.4-11) WAe001-A (WA01, H1) WAe001-A-A (H1X-10/H1.2-20) WAe001-A-2B (H1-NSD2-/-) WAe001-A-B (H1X-59/H1.2-6) WAe001-A-C (H1X-10/H1.3-62) WAe001-A-D (H1X-59/H1.3-68) WAe001-A-86 (H1.139, H1.1-39) WAe001-A-72 (H1-FTO-Knockout-13) WAe001-A-31 (H1_RB1ex1_D6) WAe001-A-32 (H1_RB1ex1_E9) WAe001-A-87 (H1.213, H1.2-13) WAe001-A-G (H1X-10/H1.5-1) WAe001-A-88 (H1.2-124, H1.2124) WAe001-A-1N (H1-FIS1-KO, WA01-FIS1-KO) WAe001-A-89 (H1.3-64, H1.364) WAe001-A-H (H1X-59/H1.5-33) WAe001-A-1O (WA01-MYB-KO, H1-MYB-KO) WAe001-A-77 (H1 RYBP-FLAG-HA KI) WAe001-A-90 (H1.3-191, H1.3191) WAe001-A-I (H1.0-29/H1.1-13) WAe001-A-41 (H1-RNF2-KO-1#) WAe001-A-J (H1.0-102/H1.1-34) WAe001-A-42 (H1-RNF2-KO-2#) WAe001-A-K (H1.0-29/H1.2-31) WAe001-A-1 (H1.1, WA01.1) WAe001-A-L (H1.0-102/H1.2-6) WAe001-A-M (H1.0-29/H1.3-22) WAe001-A-N (H1.0-102/H1.3-90) WAe001-A-93 (H1.5-23, H1.523) WAe001-A-78 (H1X10, H1X-10) WAe001-A-94 (H1.528, H1.5-28) WAe001-A-Q (H1.0-29/H1.5-6) WAe001-A-73 (H1-GATAD2B-KO-79#) WAe001-A-R (H1.0-102/H1.5-44) WAe001-A-10 (H1_RB1ex3_G3) |
| Last update | 18th December 2022 |
| Notes | Linker histones H1.3/H1.4 double knockout. The H1.4 linker histone was knocked out from the WAe001-A-89 (H1.3 single knockout) subclone. |
| User feedback | |
Provider |
|
| Generator | King Abdullah University of Science and Technology (KAUST) |
| Owner | King Abdullah University of Science and Technology (KAUST) |
| Distributors | |
| Derivation country | Saudi Arabia |
External Databases |
|
| BioSamples | SAMEA112250947 |
| Cellosaurus | CVCL_C9L5 |
| Wikidata | Q123033799 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has brother:
|
External Databases (Donor) |
|
| BioSamples | SAMEA7767418 |
Ethics
Also have a look at the ethics information for the parental line
WAe001-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe001-A.
|
Culture Conditions
| Surface coating | Laminin |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| CO2 Concentration | 5 % |
| Medium | Essential 8™ |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
|||||
| NANOG |
Yes |
|||||
| OCT3 |
Yes |
|||||
| OCT4 |
Yes |
|
Morphology pictures
morphology H1.3-64-H1.4-15.pdf
pluripotent morphology
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| FOXA2 |
Yes |
| SOX17 |
Yes |
Morphology
H1.3-64-H1.4-15 Endoderm differentiation.pdf
endodermal differentiation
Protocol or reference
differentiation protocol.pdf
trilineage direct differentiation
In vitro directed differentiation
| Marker | Expressed |
| Brachyury |
Yes |
| NCAM |
Yes |
Morphology
H1.3-64-H1.4-15 Mesoderm differentiation.pdf
mesodermal differentiation
In vitro directed differentiation
| Marker | Expressed |
| Nestin |
Yes |
| SOX1 |
Yes |
| CD44 |
Yes |
Morphology
H1.3-64-H1.4-15 Ectoderm differentiation.pdf
ectodermal differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
|
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