Generation of a DKK1 homozygous knockout human embryonic stem cell line WAe001-A-21 using CRISPR/Cas9

Summary

Dickkopf1 (DKK1) is a secreted inhibitor for the Wnt signalling, which is involved in cell proliferation, tissue regeneration and embryonic development. Using CRISPR/Cas9 editing, we established a homozygous mutant DKK1 human embryonic stem cell line (WAe001-A-21). It has a 41 bp deletion in exon 2 of DKK1, leading to its coding frame shift. The WAe001-A-21 cell line maintains a normal karyotype, pluripotency markers, typical stem cell morphology and the ability to differentiate into three germ layers. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Yang Z, Yuan F, Wang N, Liu Y, Zhang J, Wei R, Wu F, Wu Y, You K, Chen Y, Yang F, Li YX
Journal Stem cell research
Publication Date 2021 Jan 8;51:102161
PubMed 33461039
DOI 10.1016/j.scr.2021.102161

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