Generation of a DKK1 homozygous knockout human embryonic stem cell line WAe001-A-21 using CRISPR/Cas9
Summary
Dickkopf1 (DKK1) is a secreted inhibitor for the Wnt signalling, which is involved in cell proliferation, tissue regeneration and embryonic development. Using CRISPR/Cas9 editing, we established a homozygous mutant DKK1 human embryonic stem cell line (WAe001-A-21). It has a 41 bp deletion in exon 2 of DKK1, leading to its coding frame shift. The WAe001-A-21 cell line maintains a normal karyotype, pluripotency markers, typical stem cell morphology and the ability to differentiate into three germ layers. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Yang Z, Yuan F, Wang N, Liu Y, Zhang J, Wei R, Wu F, Wu Y, You K, Chen Y, Yang F, Li YX |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Mar;51:102161 |
| PubMed | 33461039 |
| DOI | 10.1016/j.scr.2021.102161 |