hINS-T2A-H2B-Cherry (+/-), HMGUi001-A-1

General#

Cell Line

hPSCreg Name HMGUi001-A-1
Alternative name(s)
hINS-T2A-H2B-Cherry (+/-), HMGUi001-A-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Helmholtz Zentrum München (HMGU)
Owner Institute of Diabetes and Regeneration Research (IDR)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA5696687
Cellosaurus CVCL_WJ50

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5696688

Ethics#

Also have a look at the ethics information for the parental line HMGUi001-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Invitrogen
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

The source cell information can be found in the parental cell line HMGUi001-A.
Passage number reprogrammed 17

Reprogramming method

Vector type Integrating
Vector Plasmid
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
No
Methods used
Immunostaining, PCR, Sequencing

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones GFP Expression-positive, FACS sorting, colony picking from single cell seeding
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS iPS-Brew XF, human
Main protein source: xeno-free
Serum concentration: 0 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Nestin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
SM22-alpha
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
insulin (target)
Gene knock-in
chromosome 11, insulin gene, exon 3
Please explain briefly the supporting evidence
CRISPR-associated (CRISPR/Cas) System