Generation of FCGR3A-EGFP knock-in reporter human embryonic stem cell line, WAe001-A-3S, using CRISPR/Cas9n-based gene targeting
Summary
Fc gamma receptor IIIA (FCGR3A) encodes CD16a, a key mediator of antibody-dependent cellular cytotoxicity (ADCC) that regulates innate and adaptive immunity, especially in natural killer (NK) cells and monocytes. We generated an FCGR3A-EGFP knock-in human embryonic stem cell (hESC) line via CRISPR/Casn9n. The cell line showed a normal karyotype, maintained expression ofthe pluripotency markers OCT4, SOX2, and NANOG, and retained trilineage differentiation potential. This reporter line enables real-time tracking of FCGR3A expression during immune cell differentiation, serving as a useful tool for studying FCGR3A+ immune cell development and related immune mechanisms. Copyright © 2026 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Dong Q, Luo S, Sun J, Liu H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2026 Jul 15;95:104061 |
| PubMed | 42470827 |
| DOI | 10.1016/j.scr.2026.104061 |