Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system
Summary
Protein phosphatase 2A (PPP2CA) is one of the four main Ser/Thr phosphatase enzymes, which involved in the negative control of cell growth and division. PPP2CA is the main protein phosphatase in the heart, which regulates Ca (2+) through a series of ion channels and transporters. In this study, we generated a PPP2CA homozygous knockout human embryonic stem cell line WAe009-A-25 based on the transient expression CRISPR/Cas9 system to investigate functional effect of PP1 deficiency. This cell line has multidirectional differentiation potential, normal karyotypic and trilineage differentiation potential in vivo. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ding T, Gao Z, Liu H, Li Y, Liu S, Jian L |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Mar;67:103029 |
| PubMed | 36724553 |
| DOI | 10.1016/j.scr.2023.103029 |