Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9
Summary
The Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is a alpha subunit of the PP1 complex, which known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Here, a PPP1CA knockout human embryonic stem cell line, WAe009-A-B, was generated using the CRISPR/Cas9 system to further study the function of PPP1CA. The cell line confirmed with pluripotency, normal karyotype and differentiation potential. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wang X, Sun J, Fu C, Liu C |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Jun;69:103077 |
| PubMed | 36965407 |
| DOI | 10.1016/j.scr.2023.103077 |