General

Cell Line

hPSCreg name WAe009-A-B
Cite as:
WAe009-A-B (RRID:CVCL_C9H0)
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
Donor diseases:
Huntington's disease
WAe009-A-1P
(IsoHD-30Q-NGN2)
Donor diseases:
Huntington's disease
WAe009-A-1Q
(IsoHD-65Q-NGN2)
Donor diseases:
Huntington's disease
WAe009-A-1R
(IsoHD-81Q-NGN2)
Donor diseases:
Huntington's disease
WAe009-A-1H
(MYL3-KO)
Donor diseases:
Huntington's disease
WAe009-A-1I
(H9::GFP cyto-reporter line)
Donor diseases:
Huntington's disease
WAe009-A-1J
(H9::mcherry cyto-reporter line)
Donor diseases:
Huntington's disease
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
Donor diseases:
Huntington's disease
WAe009-A-36
(JPH2-KO)
Donor diseases:
Huntington's disease
WAe009-A-12
(H9_RB1ex3_C7)
Donor diseases:
Huntington's disease
WAe009-A-13
(H9_RB1ex3_G12LS)
Donor diseases:
Huntington's disease
WAe009-A-1E
(TBX20-KO)
Donor diseases:
Huntington's disease
WAe009-A-37
(H9-GSX2-tTA:GFP)
Donor diseases:
Huntington's disease
WAe009-A-58
(COL4A5 heterozygote)
Donor diseases:
Huntington's disease
WAe009-A-59
(dHAND KO)
Donor diseases:
Huntington's disease
WAe009-A-60
(H9 iNK5A)
Donor diseases:
Huntington's disease
WAe009-A-77
(H9-GFP)
Donor diseases:
Huntington's disease
WAe009-A-78
(TBX18-KO)
Donor diseases:
Huntington's disease
WAe009-A-7
(H9-mHOXA9)
Donor diseases:
Huntington's disease
WAe009-A-99
(dCas9-p300 H9 21)
Donor diseases:
Huntington's disease
Last update 10th October 2022
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Provider

Generator Zhengzhou Central Hospital Affiliated (ZZCH)

External Databases

BioSamples SAMEA111450648
Cellosaurus CVCL_C9H0
Wikidata Q123033861

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • HD
  • Huntington disease
  • Huntington's chorea
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 37 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Genetic modifications
PPP1CA (target)
Gene knock-out
11q13.2
CRISPR-associated (CRISPR/Cas) System