hPSCreg®
This website uses cookies to keep track of login sessions and for internal Matomo Statistics. See our Privacy Policy for details. This website uses cookies, learn more


ICGi022-A-9

Registration Summary:

K7-4Lf MT21

ICGi022-A-9

General

Cell Line
hPSCreg name ICGi022-A-9
Cite as:
ICGi022-A-9 (RRID:CVCL_E9BB)
Alternative name(s)
K7-4Lf MT21
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 3rd December 2024
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider
Generator Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Owner Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Distributors
Derivation country Russia

External Databases
BioSamples SAMEA117402930
Cellosaurus CVCL_E9BB

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.
Healthy Subject
The donor is not affected.
Synonyms
  • Healthy Subject
  • Healthy Volunteer
  • healthy volunteer

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
https://www.ncbi.nlm.nih.gov/sra/?term=SRR11413027
No disease associated mutation found

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMN14446266

Ethics

Also have a look at the ethics information for the parental line ICGi022-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line ICGi022-A.

Reprogramming method
Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming
Type of used vector free reprogramming factor(s)
None

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Gelatin
Feeder cells Mouse embryonic fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: KnockOut DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
GlutaMAX 2 mM
NEAA 0.1 mM
2-mercaptoethanol 0.1 mM
Penicillin-Streptomy 100 U/ml
rhFGF basic 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
–
–
SSEA-4
Yes
–
SOX2
Yes
–
–
NANOG
Yes
–
TRA 1-60
Yes
–
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CK18
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
CD29
Yes
Actin, alpha 2, smooth muscle, aorta
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes
Morphology

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
Karyotyping and G-banding show ICGi022-A-9 iPSCs have a normal 46,XX karyotype at passage 10.
k7-4lf-mt_2_10.jpg
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
AAVS1 (target)
Transgene expression
19q13
CRISPR-associated (CRISPR/Cas) System