General

Cell Line
hPSCreg name	ICGi021-A-2
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	ICGi021-A-2
Alternative name(s)	K6-4fpCyto-16
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	ICGi021-A-1 (K6-4fpCyto-13) ICGi021-A-3 (K6-4fpCyto-19) ICGi021-A (K6-4f) MRIi001-A-1 (C6-AAVS1-iCasRx) WAe009-A-76 WAe009-A-1A WAe009-A-1B UCSFi001-A-12 (AICS-0036-006) CRMi003-A-2 CRMi003-A-3 (Proliving, GMNN-mScarletI Reporter) MRIi003-A-7 (HK-AAVS1-iCasRx) MRIi003-A-8 (HK-AAVS1-CAG-eGFP-homo) UCSFi001-A-23 (AICS-0054-091) UCSFi001-A-36 (AICS-0036-028) BIHi001-A-1 (iBCRT Cas9v1-3G-Kl.16) ICGi015-B-1 (m6.7pCyto-17) Donor diseases: Parkinson Disease ICGi015-B-2 (m6.7pCyto-21) Donor diseases: Parkinson Disease ICGi015-B-3 (m6.7pCyto-24) Donor diseases: Parkinson Disease ICGi034-A-1 (PD30-XBP-RFP-6, PD30-4-7-XBP-RFP-6) Donor diseases: obsolete_Parkinson's disease ICGi034-A-2 (PD30-4-7-XBP-RFP-51, PD30-XBP-RFP-51) Donor diseases: obsolete_Parkinson's disease ICGi034-A-3 (PD30-4-7-XBP-RFP-52, PD30-XBP-RFP-52) Donor diseases: obsolete_Parkinson's disease ICGi034-A-4 (PD30-4-7-XBP-RFP-86, PD30-XBP-RFP-86) Donor diseases: obsolete_Parkinson's disease
Last update	16th June 2021
Notes	Healthy human iPSC clone with doxycycline-inducible expression of cytosolic Grx1-roGFP2
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Provider
Generator	Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Owner	Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Derivation country	Russia
External Databases
BioSamples	SAMEA9218647
General Information
* Is the cell line readily obtainable for third parties?	Yes Cell line can only be used in: Any Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	ICGi021-A

Donor Information

General Donor Information
Sex	female
Ethnicity	Caucasian
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	Yes Exome sequencing https://www.ncbi.nlm.nih.gov/sra/?term=SRR11413028 No disease associated mutation found
External Databases (Donor)
BioSamples	SAMEA6983569

Ethics

Also have a look at the ethics information for the parental line ICGi021-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line ICGi021-A.
Reprogramming method
Vector type	Non-integrating
Vector	Episomal
Is reprogramming vector detectable?	No
Methods used	PCR
Files and images showing reprogramming vector expressed or silenced	episomes.tif
Vector free reprogramming
Type of used vector free reprogramming factor(s)	None
Other
Derived under xeno-free conditions	No
Derived under GMP?	No
Available as clinical grade?	No

Culture Conditions

Surface coating	Gelatin
Feeder cells	MEF cell Cellfinder Ont Id: http://www.ebi.ac.uk/efo/EFO
Passage method	Enzymatically TrypLE
O2 Concentration	20 %
CO2 Concentration	5 %
Medium	Other medium: Base medium: Knock-out DMEM Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements Supplement Amount Unit GlutaMAX 2 mM NEAA 0.1 mM Penicillin-Streptomycin 100 U/ml 2-mercaptoethanol 0.1 mM rhFGF basic 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
POU5F1 (OCT-4)	Yes	–
SSEA-4	Yes	–
Alkaline Phosphatase	Yes				–

Morphology

Morphology pictures

Morphol K64f Cyto16.tif

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Marker	Expressed
CK-18	Yes
GATA6	Yes

Morphology

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

In vitro directed differentiation

Marker	Expressed
Collagen type I	Yes
aSMA	Yes

Morphology

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

In vitro directed differentiation

Marker	Expressed
Tubulin, beta 3	Yes
Neurofilament 200	Yes

Morphology

Microbiology / Virus Screening
Mycoplasma	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46,XX,del(9)(q12q21.1) №_4_206.pdf Passage number: 8 Karyotyping method: G-Banding
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications	Healthy Synonyms Healthy Well Genetic modifications
Genetic modifications not related to a disease	AAVS1 (target) Type of modification Transgene expression Chromosome location 19q13 Free text Transgenes of glutathione redox sensor (cytosolic) cyto_Grx1-roGFP2 and tetracycline-transactivator (rtTA) in AAVS1 locus were detected by PCR. Delivery method CRISPR-associated (CRISPR/Cas) System Available documents puro in.TIF cyto Grx1-roGFP2 transgene detection neo in.TIF rtTA transgene detection