WAe009-A-45
General
Cell Line |
|
hPSCreg Name | WAe009-A-45 |
Cell line type | Human embryonic stem cell (hESC) |
Similar lines |
WAe009-A (WA09, H9) DHMi005-A-5 (L_mut_FLAG Clone 4) DHMi005-A-6 (L_mut_FLAG Clone 14) DHMi005-A-7 (L_mut_FLAG Clone 15) WAe009-A-62 (KCNQ1 KO) WAe009-A-58 (COL4A5 heterozygote) WAe009-A-77 (H9-GFP) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-99 (dCas9-p300 H9 21) WAe009-A-1G (ISL1-KO) WAe009-A-90 (H9_LCCS1) WAe009-A-72 (COL1A2 -/-) WAe009-A-93 (NIS-EGFP-Fluc H9) |
Last update | 22nd January 2021 |
User feedback | |
Provider |
|
Generator | gynaecology and obstetrics, Anzhen Hospital (GOAH) |
Owner | gynaecology and obstetrics, Anzhen Hospital (GOAH) |
Distributors | |
Derivation country | China |
External Databases |
|
Cellosaurus | CVCL_A1MY |
CLO | CLO_0103111 |
BioSamples | SAMEA8072991 |
Wikidata | Q102115104 |
General Information |
|
Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Subclone of |
Donor Information
General Donor Information |
|
Sex | female |
Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Family history | NO |
Is the medical history available upon request? | NO |
Is clinical information available? | NO |
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
Other cell lines of this donor | |
External Databases (Donor) |
|
BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
Is there an MTA available for the cell line? | No |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
|||||
SSEA-4 |
Yes |
Differentiation Potency
Microbiology / Virus Screening |
|
Mycoplasma | Negative |
Certificate of Analysis |
|
Is there a certificate of analysis available? |
Yes
Passage:
30
|
Genotyping
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
|
Login to share your feedback, experiences or results with the research community.