L_mut_FLAG Clone 14
DHMi005-A-6
General
Cell Line |
|
| hPSCreg name | DHMi005-A-6 |
| Cite as: | DHMi005-A-6 (RRID:CVCL_D0E1) |
| Alternative name(s) |
L_mut_FLAG Clone 14
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
DHMi005-A-5 (L_mut_FLAG Clone 4) DHMi005-A-7 (L_mut_FLAG Clone 15) DHMi005-A (Control_L) DHMi005-A-1 (L_mut) DHMi005-A-2 (L_FLAG Clone 5) DHMi005-A-3 (L_FLAG Clone 6) DHMi005-A-4 (L_FLAG Clone 7) DHMi005-A-8 (L Delta B2M Clone 6) DHMi005-A-9 (L Delta B2M Clone 11) ITXi006-A-1 (IM-R406W) UCSFi001-A-49 (YH653-MUT-1C8-HET, DYT1-HET-1C8) UCSFi001-A-50 (YH653-MUT-H6-HET, DYT1-HET-H6) UCSFi001-A-51 (YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2) BIONi010-C-10 (HNF1AP291fsinsC +/- 54-5) BIONi010-C-11 (HNF1AP291fsinsC -/- 66-1) BIONi010-C-12 (HNF4ApR309C -/- 2-4) BIONi010-C-65 (BiONI010-C-O16) ICANi002-A-5 (ICAN-PKP2-H695-W11) BIONi010-C-66 (BIONi010-C-N7) |
| Last update | 20th October 2022 |
| User feedback | |
Provider |
|
| Generator | German Heart Center Munich (DHM) |
| Owner | German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure |
| Distributors | |
| Derivation country | Germany |
External Databases |
|
| BioSamples | SAMEA111493346 |
| Cellosaurus | CVCL_D0E1 |
| Wikidata | Q123031167 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
| Family history | No abnormalities have been described with regard to the family history. |
| Is the medical history available upon request? | no, healthy proband |
| Is clinical information available? | no, healthy proband |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA12738677 |
Ethics
Also have a look at the ethics information for the parental line
DHMi005-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Genscript, Addgene |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
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The source cell information can be found in the parental cell line
DHMi005-A.
|
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Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
ReLeSR
|
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XY
Passage number: 70
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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