IM-R406W
ITXi006-A-1
General
Cell Line |
|
| hPSCreg name | ITXi006-A-1 |
| Cite as: | ITXi006-A-1 |
| Alternative name(s) |
IM-R406W
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
ITXi006-A (WT8288) UCSFi001-A-49 (YH653-MUT-1C8-HET, DYT1-HET-1C8) UCSFi001-A-50 (YH653-MUT-H6-HET, DYT1-HET-H6) UCSFi001-A-51 (YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2) ICANi002-A-5 (ICAN-PKP2-H695-W11) DHMi005-A-5 (L_mut_FLAG Clone 4) DHMi005-A-1 (L_mut) DHMi005-A-6 (L_mut_FLAG Clone 14) DHMi005-A-7 (L_mut_FLAG Clone 15) BIONi010-C-10 (HNF1AP291fsinsC +/- 54-5) BIONi010-C-11 (HNF1AP291fsinsC -/- 66-1) BIONi010-C-12 (HNF4ApR309C -/- 2-4) BIONi010-C-65 (BiONI010-C-O16) BIONi010-C-66 (BIONi010-C-N7) |
| Last update | 22nd January 2024 |
| User feedback | |
Provider |
|
| Generator |
l’institut du thorax (ITX)
Contact:
l’institut du thorax (ITX) |
| Owner | l’institut du thorax (ITX) |
| Distributors | |
| Derivation country | France |
External Databases |
|
| BioSamples | SAMEA115121928 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | European |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
Normal male karyotype with no aneuploidies detected |
External Databases (Donor) |
|
| BioSamples | SAMEA11285999 |
Ethics
Also have a look at the ethics information for the parental line
ITXi006-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
ITXi006-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: iPS Brew
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
|
Genetic Modification
| Disease/phenotype related modifications |
|
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