WTSIi018-B-29

DU414

The cell line is not validated yet.

General

Cell Line

hPSCreg name WTSIi018-B-29
Cite as:
WTSIi018-B-29
Alternative name(s)
DU414
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 23rd April 2025
Notes Cell line was generated from the subclone WTSIi018-B-12
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Provider

Generator IUF – Leibniz Research Institute for Environmental Medicine (IUF)
Owner IUF – Leibniz Research Institute for Environmental Medicine (IUF)
Derivation country Germany

External Databases

BioSamples SAMEA117987740

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398402
HipSci HPSI-kolf

Ethics

Also have a look at the ethics information for the parental line WTSIi018-B .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution

hIPSC Derivation

General

The source cell information can be found in the parental cell line WTSIi018-B.

Reprogramming method

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Morphology pictures
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Data analysis report
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Protocol or reference
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Protocol or reference
DU414 fatescore.png
QPCR based hiPSCore analysis for ectoderm markers

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 14
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
DMD pathogenic Nonsense mutation c.5713G>T(p.Arg1905Ter)
Synonyms
  • DMD
  • Duchenne muscular dystrophy
  • Duchenne muscular dystrophy, X-linked recessive
  • muscular dystrophy, Duchenne
  • severe dystrophinopathy, Duchenne type
  • muscular dystrophy, Duchenne type
  • muscular dystrophy, pseudohypertrophic progressive, Duchenne type
show more synonyms
Genetic modifications
Gene knock-in
Chromosome Xp21.1
c.5713G>T Base substitution using a single stranded donor oligo with indented point mutation and silent mutations
CRISPR-associated (CRISPR/Cas) System