HPSI0114i-kolf_2-C1

General#

Cell Line

hPSCreg Name WTSIi018-B-1
Alternative name(s)
HPSI0114i-kolf_2-C1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 25th October 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Owner Wellcome Trust Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

HipSci HPSI0114i-kolf_2-C1
Cellosaurus CVCL_9S58

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Non-disease associated phenotypes
  • Healthy

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398402
HipSci HPSI-kolf

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line WTSIi018-B.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Methods used
Immunostaining

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
No

Culture Conditions#

Feeder cells
No
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GDF3
Yes
nodal
Yes
podxl
Yes
xist
Yes
LEFTY1
Yes
foxd3
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes

Genetic Modification#