HPSI0114i-kolf_2-C1
WTSIi018-B-1
General
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
| Non-disease associated phenotypes |
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA2398402 |
| HipSci | HPSI-kolf |
Ethics
Also have a look at the ethics information for the parental line
WTSIi018-B
.
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Lifetech |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | can only be used for academic reserach not commercial |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
WTSIi018-B.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Methods used |
Immunostaining
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
No |
Culture Conditions
| Feeder cells |
No |
| Medium |
TeSR™ E8™
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| nodal |
Yes |
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| podxl |
Yes |
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| xist |
Yes |
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| LEFTY1 |
Yes |
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| foxd3 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
normal
Passage number: 30
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
|
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