DU411
The cell line is not validated yet.
WTSIi018-B-28
General
Cell Line |
|
| hPSCreg name | WTSIi018-B-28 |
| Cite as: | WTSIi018-B-28 |
| Alternative name(s) |
DU411
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 3rd June 2025 |
| Notes | Cell line was generated from the subclone WTSIi018-B-12 |
| User feedback | |
Provider |
|
| Generator | IUF – Leibniz Research Institute for Environmental Medicine (IUF) |
| Owner | IUF – Leibniz Research Institute for Environmental Medicine (IUF) |
| Derivation country | Germany |
External Databases |
|
| BioSamples | SAMEA117987737 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA2398402 |
| HipSci | HPSI-kolf |
Ethics
Also have a look at the ethics information for the parental line
WTSIi018-B
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
WTSIi018-B.
|
|
Reprogramming method |
|
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| O2 Concentration | 10 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
Project_d2.2 dmd_ch00.tif
DMD DU411
The hiPSCore is a machine learning-based thoroughly validated functional pluripotency test (Dobner et al, 2024). By analyzing hiPSC-derived
undifferentiated, endoderm-, ectoderm-, and mesodermdifferentiated samples using qPCR, the automated hiPSCore classification assesses differentiation potential of hiPSCs with high accuracy, sensitivity, and specificity. Successfully
validated cell lines are truly pluripotent according to the Standards of the International Society for Stem Cell Research (ISSCR) and may be used for research. The attached report contains detailed analysis and description of the results. The attached plot shows the scoring of each of the four differentiation states and the resulting hiPSCore (> 4: PASS).
Method documentation
2025-06-03_hiPSCore - cell fate.png
hiPSCore cell fate plot
DU411_hiPSCore Report.pdf
hiPSCore cell line report
Differentiation Potency
In vitro directed differentiation
Scorecard
Protocol or reference
In vitro directed differentiation
Scorecard
Protocol or reference
In vitro directed differentiation
Scorecard
Protocol or reference
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
|
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