CEBe033-A-8

SA001_KO_HGPRT_1

General

Cell Line

hPSCreg name CEBe033-A-8
Cite as:
CEBe033-A-8 (RRID:CVCL_C9YW)
Alternative name(s)
SA001_KO_HGPRT_1
Cell line type Human embryonic stem cell (hESC)
Similar lines
CEBe033-A-10
(SA001_WT_HGPRT_1)
CEBe033-A-11
(SA001_WT_HGPRT_2)
CEBe033-A
(SA001)
CEBe033-A-9
(SA001_KO_HGPRT_2)
CEBe033-A-1
(GBA1_hESC_GBA#1-/-_10.18.11)
CEBe033-A-2
(SA001 Shank3 hetero1)
CEBe033-A-3
(SA001 Shank3 Hetero2)
CEBe033-A-4
(SA001 Shank3 Hetero3)
CEBe033-A-5
(SA001 Shank3 Homo1)
CEBe033-A-6
(SA001 Shank3 Homo2)
CEBe033-A-7
(SA001 Shank3 Homo3)
BIONi010-C-10
(HNF1AP291fsinsC +/- 54-5)
BIONi010-C-11
(HNF1AP291fsinsC -/- 66-1)
BIONi010-C-12
(HNF4ApR309C -/- 2-4)
Last update 27th April 2023
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Provider

Generator Institut from Stem cell Therapy and Exploration of Monogenic diseases (ISTEM)
Derivation country France

External Databases

BioSamples SAMEA112964584
Cellosaurus CVCL_C9YW
Wikidata Q123030893

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal, 46XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104132810

Ethics

Also have a look at the ethics information for the parental line CEBe033-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution Work with human embryonic stem cells requires prior approval by local ethics committees

hESC Derivation

The source cell information can be found in the parental cell line CEBe033-A.

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMacs iPS Brews
Main protein source:
Serum concentration: %
Supplements
StemMacs iPS Brews supp 10 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
NANOG
Yes
TRA 1-81
Yes
SSEA-3
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2
Yes
Brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TuJ1
Yes
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY
Passage number: 79
Karyotyping method: Spectral karyotyping

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
No mutations

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • HPRT complete deficiency
  • Hypoxanthine guanine phosphoribosyltransferase complete deficiency
  • HPRT deficiency grade IV
  • Hypoxanthine guanine phosphoribosyltransferase deficiency, grade IV
show more synonyms
Genetic modifications
HPRT1 (target)
Gene knock-out
Xq26.2-q26.3
Mutation on the HGPRT gene located of the X chromosome (ChrX :134498209-134498231)
CRISPR-associated (CRISPR/Cas) System