CEBe033-A-1

GBA1_hESC_GBA#1-/-_10.18.11

General#

Cell Line

hPSCreg Name CEBe033-A-1
Alternative name(s)
GBA1_hESC_GBA#1-/-_10.18.11
Cell line type Human embryonic stem cell (hESC)
Last update 8th September 2017
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Provider

Generator F. Hoffmann-La Roche Ltd (ROCHE)
Owner F. Hoffmann-La Roche Ltd (ROCHE)

External Databases

BioSamples SAMEA104132790
Cellosaurus CVCL_QX50

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
X,Y 46
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA104132810

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2013-11-11
Supernumerary embryos from IVF treatment?
No
Other hESC source: SA001 parental line
PGD Embryo?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 19 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Unkown
Differentiation Potency
Neuron
Ont Id: CL_0000540
Neuroepithelial Stem Cell
Ont Id: CL_0002259

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
X,Y, 46

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Gaucher disease
targeted insertion of disruption cassettes (pGK-neo, pGK-puro) into exon 4 of both alleles
Synonyms
  • Glucocerebrosidase deficiency
  • Acid beta-glucosidase deficiency
Genetic modifications
GBA (target)
Gene knock-in
1q22
1q22
Zinc finger nuclease