SA001_WT_HGPRT_2
CEBe033-A-11
General
Cell Line |
|
| hPSCreg name | CEBe033-A-11 |
| Cite as: | CEBe033-A-11 (RRID:CVCL_C9Z5) |
| Alternative name(s) |
SA001_WT_HGPRT_2
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
CEBe033-A-8 (SA001_KO_HGPRT_1) CEBe033-A-10 (SA001_WT_HGPRT_1) CEBe033-A (SA001) CEBe033-A-9 (SA001_KO_HGPRT_2) CEBe033-A-1 (GBA1_hESC_GBA#1-/-_10.18.11) CEBe033-A-2 (SA001 Shank3 hetero1) CEBe033-A-3 (SA001 Shank3 Hetero2) CEBe033-A-4 (SA001 Shank3 Hetero3) CEBe033-A-5 (SA001 Shank3 Homo1) CEBe033-A-6 (SA001 Shank3 Homo2) CEBe033-A-7 (SA001 Shank3 Homo3) BIONi010-C-10 (HNF1AP291fsinsC +/- 54-5) BIONi010-C-11 (HNF1AP291fsinsC -/- 66-1) BIONi010-C-12 (HNF4ApR309C -/- 2-4) |
| Last update | 27th April 2023 |
| Notes | Subclone was generated without CRISPR/Cas9 RNP and that it is an internal control (isogenic cell line for CEBe033-A8 and CEBe033-A9) |
| User feedback | |
Provider |
|
| Generator | Institut from Stem cell Therapy and Exploration of Monogenic diseases (ISTEM) |
| Derivation country | France |
External Databases |
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| BioSamples | SAMEA112966293 |
| Cellosaurus | CVCL_C9Z5 |
| Wikidata | Q123030885 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA104132810 |
Ethics
Also have a look at the ethics information for the parental line
CEBe033-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | Work with human embryonic stem cells requires prior approval by local ethics committees |
hESC Derivation
|
The source cell information can be found in the parental cell line
CEBe033-A.
|
Culture Conditions
| Surface coating | Vitronectin | ||||||
| Feeder cells |
No |
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| Passage method | Mechanically | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: StemMacs iPS Brews
Main protein source: Serum concentration: % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
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| NANOG |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-3 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
No mutations |
Genetic Modification
| Disease/phenotype related modifications |
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