SA001

General#

Cell Line

hPSCreg Name CEBe033-A
Alternative name(s)
SA001
Cell line type Human embryonic stem cell (hESC)
Last update 8th September 2017
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Provider

Generator Cellartis AB Sweden (CEB)
Derivation country Sweden

External Databases

Cellosaurus CVCL_B347
NIHhESC NIHhESC-10-0085

General Information

Publications
Projects
Is the cell line available in principle?
Yes
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA104132810

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2001-03-20
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells MEF
Cellfinder Ont Id: C57BL/6
Medium VitroHES
Supplements
hrbFGF %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
NANOG
Yes
DNMT3B
Yes
GABRB3
Yes
TDGF1
Yes
Telomerase Activity
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)