SA001

General

Cell Line

hPSCreg name CEBe033-A
Cite as:
CEBe033-A (RRID:CVCL_B347)
Alternative name(s)
SA001
Cell line type Human embryonic stem cell (hESC)
Similar lines
CEBe033-A-11
(SA001_WT_HGPRT_2)
CEBe033-A-1
(GBA1_hESC_GBA#1-/-_10.18.11)
CEBe033-A-2
(SA001 Shank3 hetero1)
CEBe033-A-3
(SA001 Shank3 Hetero2)
CEBe033-A-4
(SA001 Shank3 Hetero3)
CEBe033-A-5
(SA001 Shank3 Homo1)
CEBe033-A-8
(SA001_KO_HGPRT_1)
CEBe033-A-6
(SA001 Shank3 Homo2)
CEBe033-A-9
(SA001_KO_HGPRT_2)
CEBe033-A-7
(SA001 Shank3 Homo3)
CEBe033-A-10
(SA001_WT_HGPRT_1)
Last update 20th April 2023
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Provider

Generator Takara Bio Europe AB (former Cellartis) (CEB)
Derivation country Sweden

External Databases

Cellosaurus CVCL_B347
NIHhESC NIHhESC-10-0085
Wikidata Q54951989
BioSamples SAMEA112937558

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclones

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104132810

Ethics

Was the embryo established purely for research purposes? Yes
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information catharina_brandsten@takarabio.com
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-10-0085
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? EKBB Ethikkommisson beider Basel Hebelstrasse 53 4056 Basel
Approval number R-FP-S-2-0006-0006

hESC Derivation

Date of derivation 2001-03-20
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Feeder cells MEF
Cellfinder Ont Id: C57BL/6
Medium VitroHES
Supplements
hrbFGF %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
NANOG
Yes
DNMT3B
Yes
GABRB3
Yes
TDGF1
Yes
Telomerase Activity
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 52

Other Genotyping (Cell Line)