SA001 DP427 KO1
The cell line is not validated yet.
CEBe033-A-12
General
Cell Line |
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hPSCreg name | CEBe033-A-12 |
Cite as: | CEBe033-A-12 |
Alternative name(s) |
SA001 DP427 KO1
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Cell line type | Human embryonic stem cell (hESC) |
Similar lines | No similar lines found. |
Last update | 13th August 2025 |
User feedback | |
Provider |
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Generator | Institut from Stem cell Therapy and Exploration of Monogenic diseases (ISTEM) |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA104132810 |
Ethics
Also have a look at the ethics information for the parental line
CEBe033-A
.
Is there an MTA available for the cell line? | Yes |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
Constraints for use or distribution | Work with human embryonic stem cells requires prior approval by local ethics committees. |
hESC Derivation
The source cell information can be found in the parental cell line
CEBe033-A.
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Culture Conditions
Surface coating | Vitronectin | ||||||
Feeder cells |
No |
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Passage method | Mechanically | ||||||
CO2 Concentration | 5 % | ||||||
Medium |
Other medium:
Base medium: StemMacs IPS-Brew XF
Main protein source: Serum concentration: % Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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SSEA-4 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
No mutations |
Genetic Modification
Disease/phenotype related modifications |
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