WTSIi018-B-20

General

Cell Line

hPSCreg name WTSIi018-B-20
Cite as:
WTSIi018-B-20 (RRID:CVCL_D6RI)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi018-B
(HPSI0114i-kolf_2)
WTSIi018-B-21
(Kolf 2.1 J (TARDBP M337V SNV/SNV))
WTSIi018-B-1
(HPSI0114i-kolf_2-C1)
WTSIi018-B-22
(Kolf 2.1 J (TARDBP Q331K WT/SNV))
WTSIi018-B-23
(Kolf 2.1 J (TARDBP Q331K SNV/SNV))
WTSIi018-B-24
(Kolf 2.1 J (FUS R495X WT/SNV))
WTSIi018-B-25
(Kolf 2.1 J (FUS R495X SNV/SNV))
WTSIi018-B-26
(Kolf 2.1 J (FUS R521H WT/SNV))
WTSIi018-B-27
(Kolf 2.1 J (FUS R521H SNV/SNV))
WTSIi018-B-18
(Kolf 2.1 J (TARDBP M337V WT/SNV))
WTSIi018-A
(HPSI0114i-kolf_3)
Last update 4th March 2024
Notes Cell line was generated from the subclone WTSIi018-B-12
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Provider

Generator IUF – Leibniz Research Institute for Environmental Medicine (IUF)
Owner IUF – Leibniz Research Institute for Environmental Medicine (IUF)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA115295915
Cellosaurus CVCL_D6RI

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398402
HipSci HPSI-kolf

Ethics

Also have a look at the ethics information for the parental line WTSIi018-B .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution

hIPSC Derivation

General

The source cell information can be found in the parental cell line WTSIi018-B.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
ACTB Loss-of-Function Mutation
Synonyms
  • Inactivating Gene Mutation
  • Inactivating Mutation
  • Loss of Function Gene Mutation
  • Loss of Function Mutation
show more synonyms
Genetic modifications
Gene knock-out
7p22.1
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease
3p
We found a copy-neutral loss-of-heterozygosity in chromosome 3 covering a region of approximately 90 Mbp in size by performing SNP microarray