WTSIi018-B-20
General
Cell Line |
|
| hPSCreg name | WTSIi018-B-20 |
| Cite as: | WTSIi018-B-20 (RRID:CVCL_D6RI) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
WTSIi018-B (HPSI0114i-kolf_2) WTSIi018-B-21 (Kolf 2.1 J (TARDBP M337V SNV/SNV)) WTSIi018-B-1 (HPSI0114i-kolf_2-C1) WTSIi018-B-22 (Kolf 2.1 J (TARDBP Q331K WT/SNV)) WTSIi018-B-23 (Kolf 2.1 J (TARDBP Q331K SNV/SNV)) WTSIi018-B-24 (Kolf 2.1 J (FUS R495X WT/SNV)) WTSIi018-B-25 (Kolf 2.1 J (FUS R495X SNV/SNV)) WTSIi018-B-26 (Kolf 2.1 J (FUS R521H WT/SNV)) WTSIi018-B-27 (Kolf 2.1 J (FUS R521H SNV/SNV)) WTSIi018-B-18 (Kolf 2.1 J (TARDBP M337V WT/SNV)) WTSIi018-A (HPSI0114i-kolf_3) |
| Last update | 4th March 2024 |
| Notes | Cell line was generated from the subclone WTSIi018-B-12 |
| User feedback | |
Provider |
|
| Generator | IUF – Leibniz Research Institute for Environmental Medicine (IUF) |
| Owner | IUF – Leibniz Research Institute for Environmental Medicine (IUF) |
| Distributors | |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA115295915 |
| Cellosaurus | CVCL_D6RI |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46,XY
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA2398402 |
| HipSci | HPSI-kolf |
Ethics
Also have a look at the ethics information for the parental line
WTSIi018-B
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
WTSIi018-B.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XY
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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