WTSIi018-B-14
General
Cell Line |
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| hPSCreg name | WTSIi018-B-14 |
| Cite as: | WTSIi018-B-14 (RRID:CVCL_D6KS) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
WTSIi018-B (HPSI0114i-kolf_2) WTSIi018-B-21 (Kolf 2.1 J (TARDBP M337V SNV/SNV)) WTSIi018-B-1 (HPSI0114i-kolf_2-C1) WTSIi018-B-22 (Kolf 2.1 J (TARDBP Q331K WT/SNV)) WTSIi018-B-23 (Kolf 2.1 J (TARDBP Q331K SNV/SNV)) WTSIi018-B-24 (Kolf 2.1 J (FUS R495X WT/SNV)) WTSIi018-B-25 (Kolf 2.1 J (FUS R495X SNV/SNV)) WTSIi018-B-26 (Kolf 2.1 J (FUS R521H WT/SNV)) WTSIi018-B-27 (Kolf 2.1 J (FUS R521H SNV/SNV)) WTSIi018-B-18 (Kolf 2.1 J (TARDBP M337V WT/SNV)) WTSIi018-A (HPSI0114i-kolf_3) |
| Last update | 18th December 2023 |
| User feedback | |
Provider |
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| Generator | Paul-Ehrlich-Institut (PEI) |
External Databases |
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| BioSamples | SAMEA115039273 |
| Cellosaurus | CVCL_D6KS |
| Wikidata | Q127384717 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA2398402 |
| HipSci | HPSI-kolf |
Ethics
Also have a look at the ethics information for the parental line
WTSIi018-B
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
WTSIi018-B.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Other medium:
Base medium: StemFlex™ Basal Medium
Main protein source: Serum concentration: % |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line WTSIi018-B .Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
| Disease/phenotype related modifications |
Synonyms
Genetic modifications
ATM (target)Isogenic modification
11q22.3
NM_000051.4(ATM):c.9046A>C (p.Lys3016Gln)
Homozygous
Homozygous point mutation A->C in exon 63 at K3016 in the ATM gene. Introduction of K3016Q. When DNA damage accurs Lysine 3016 of ATM get acetylated and therefore activates ATM kinase activity. Cell line was generated by using CRISPR/Cas-mediated Homology-directed Repair.
Mutated
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